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A combustible liquid created by the Still using Biomass at a 10:3 (30%) conversion ratio or, in a Refinery at a 4:1 (25%) ratio. Biofuel can be used to power Combustion Engines. Provides 5 MJ/t for 40,000 ticks (per bucket), giving it 33% the energy density of Fuel (6 MJ/t for 100,000 ticks) but 400% the energy density of Biomass (5MJ/t for 10,000 ticks in a Biogas Engine).
Creating Biofuel in a Refinery has no advantage over Biomass other than compressing the same energy into a smaller amount of liquid, since the 1:4 increase in energy density is completely offset by the 4:1 efficiency of the conversion process. A Still, however, converts 50,000 MJ worth of Biomass into 60,000 MJ worth of Biofuel, yielding a net gain even when accounting for the energy required to run the Still.
If the goal is EU rather than MJ, Biofuel can also be used in a Bio Generator for 32,000 EU at 16 EU/t (per bucket). However, when used in a Combustion Engine to power a Magma Crucible drawing Cobblestone from an Igneous Extruder and sending Lava to a Thermal Generator, a single bucket of Biofuel can produce 300,000 EU; replacing the Cobblestone with Netherrack can yield 500,000 EU per bucket. This vast improvement in efficiency comes at the expense of considerable complexity in the generator setup.
How To Make, Store, And Use Biofuel[]
This video demonstrates how to setup a full production of biofuel: